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Relationship between the expression of Rab family GTPases and neuropeptide hormones in the brain of Bombyx mori

Histochemistry and Cell Biology, ISSN: 0948-6143, Vol: 139, Issue: 2, Page: 299-308
2013
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Article Description

Rab proteins are small GTPases that play essential roles in vesicle transport. In this study, we examined the expression of Rab proteins and neuropeptide hormones in the brain of the silkworm, Bombyx mori. We produced antibodies against B. mori Rab1 and Rab14 in rabbits. Immunoblotting of samples of brain tissue from B. mori revealed a single band for each antibody. Rab1 and Rab14 immunohistochemical labeling in the brain of B. mori was restricted to neurons of the pars intercerebralis and dorsolateral protocerebrum. Rab1, Rab7 and Rab14 co-localized with bombyxin. Rab1 and Rab7 co-localized with eclosion hormone. Rab1 co-localized with prothoracicotropic hormone. These results suggest that Rab1, Rab7 and Rab14 may be involved in neuropeptide transport in the brain of B. mori. This is the first report on the specificity of Rab proteins for the secretion of different neuropeptides in insects. © 2012 Springer-Verlag.

Bibliographic Details

Uno, Tomohide; Sakamoto, Kazuki; Isoyama, Yuri; Hiragaki, Susumu; Uno, Yuichi; Kanamaru, Kengo; Yamagata, Hiroshi; Takagi, Michihiro; Mizoguchi, Akira; Takeda, Makio

Springer Science and Business Media LLC

Medicine; Biochemistry, Genetics and Molecular Biology; Health Professions

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