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Production, partial purification and characterization of a proteoglycan bioemulsifier from an oleaginous yeast

Bioprocess and Biosystems Engineering, ISSN: 1615-7605, Vol: 43, Issue: 10, Page: 1747-1759
2020
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Article Description

Abstract: In this study, Meyerozyma caribbica, an indigenously isolated oleaginous yeast, produced in media containing glucose a bioemulsifier that was partially characterized as a proteoglycan based on preliminary analysis. Optimization of carbon:nitrogen (C:N) ratio revealed 30:1 as the suitable ratio for enhanced production. Apart from higher emulsification activity (E: 70–80%), this molecule showed strong emulsion stability over a wide range of pH (2.0–9.0), salinity (0.05%—10%, w/v) and temperature (− 80 °C to + 50 °C). The current study emphasizes on the determination of critical media parameters for improved and stable bioemulsifier production coupled with partial characterization and identification of the molecule. Thus, a proteoglycan-based bioemulsifier with such a stable emulsifying property can serve as a versatile and potential component in food, cosmetics and pharmaceutical formulations. Graphic abstract: [Figure not available: see fulltext.]

Bibliographic Details

Bhaumik, Moumita; Dhanarajan, Gunaseelan; Chopra, Jayita; Kumar, RaviRanjan; Hazra, Chinmay; Sen, Ramkrishna

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology; Chemical Engineering

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