Visual and microplate detection of aflatoxin B2 based on NaCl-induced aggregation of aptamer-modified gold nanoparticles
Microchimica Acta, ISSN: 1436-5073, Vol: 182, Issue: 5-6, Page: 995-1001
2015
- 45Citations
- 34Captures
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Article Description
We describe a fast and sensitive method for the detection of aflatoxin B2 (AFB2) that is based on the addition of AFB2-sensitive aptamers to a colloidal solution of gold nanoparticles (AuNPs). Subsequent addition of AFB2 and NaCl to the solution causes a color change from wine-red to blue-purple. Best results are obtained at a pH value of 7.0, a 10 mM aptamer concentration, and a 2 M concentration of NaCl. The method has a linear dynamic range that extends from 0.025 to 10 ng∙mL of AFB2, and the detection limit is 25 pg∙mL. The method is simple, fast, highly sensitive, and enables both visual and microplate readout. [Figure not available: see fulltext.]
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84925512102&origin=inward; http://dx.doi.org/10.1007/s00604-014-1420-5; http://link.springer.com/10.1007/s00604-014-1420-5; http://link.springer.com/content/pdf/10.1007/s00604-014-1420-5; http://link.springer.com/content/pdf/10.1007/s00604-014-1420-5.pdf; http://link.springer.com/article/10.1007/s00604-014-1420-5/fulltext.html; https://dx.doi.org/10.1007/s00604-014-1420-5; https://link.springer.com/article/10.1007/s00604-014-1420-5
Springer Science and Business Media LLC
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