Gold nanoparticles functionalized with Ru(II)bipyridyl labeled DNA as a luminescent probe for the sensitive determination of DNase I
Microchimica Acta, ISSN: 1436-5073, Vol: 184, Issue: 9, Page: 3273-3279
2017
- 9Citations
- 8Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
The authors describe a fluorometric method for the determination of the activity of the enzyme DNase I. Gold nanoparticles were functionalized with a Ru(II)bipyridyl complex to which dsDNA chains were covalatently linked via carboxy groups. The emission of the Ru(II) complex (measured at excitation/emission wavlengths of 450/629 nm) is quenched by the gold nanoparticles due to surface energy transfer. The emission is restored as soon as the Ru(II)-labeled dsDNA is cut off by the action of DNase I. Based on this mechanism, the DNase I activity in vitro was detected with high sensitivity. The effect of the different lengths of DNA chains on luminescence quenching and sensing ability was studied. The intensity of restored luminescence is linearly related to the activity of DNase I in the range from 4 to 400 mU·mL with a detection limit of 50 μU·mL. [Figure not available: see fulltext.].
Bibliographic Details
Springer Science and Business Media LLC
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know