Immunoliposome-based fluorometric patulin assay by using immunomagnetic nanoparticles
Microchimica Acta, ISSN: 1436-5073, Vol: 186, Issue: 12, Page: 834
2019
- 16Citations
- 15Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations16
- Citation Indexes16
- 16
- CrossRef4
- Captures15
- Readers15
- 15
Article Description
A fluorometric immunoassay is described for the determination of patulin, a highly toxic fungal metabolite. A rabbit anti-patulin-bovine serum albumin (BSA) IgG conjugate was prepared and used to compose immunoliposomes and immunomagnetic nanoparticles. The immunomagnetic nanoparticles are then added to the sample to form the patulin-antibody composites which can be magnetically separated. The immunoliposomes are then added to form a sandwich. After magnetic separation of the composites and adding n-octyl-β-D-glucopyranoside, the fluorophore sulforhodamine B (SRB) is released. Its fluorescence intensity was then measured at excitation/emission wavelengths of 550/585 nm. The immunoliposome-based immunomagnetic nanoparticle assay can detect 8 μg L of patulin in apple juice without the need for extraction, separation, and purification. The detection limit falls within the European regulatory limit for infants and children’s products (10 μg L). The method is rapid, enviroment-friendly, and reliable. [Figure not available: see fulltext.].
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85075480087&origin=inward; http://dx.doi.org/10.1007/s00604-019-3973-9; http://www.ncbi.nlm.nih.gov/pubmed/31758269; http://link.springer.com/10.1007/s00604-019-3973-9; https://dx.doi.org/10.1007/s00604-019-3973-9; https://link.springer.com/article/10.1007/s00604-019-3973-9
Springer Science and Business Media LLC
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