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Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016

Archives of Virology, ISSN: 1432-8798, Vol: 166, Issue: 11, Page: 3165-3172
2021
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Article Description

This study combined conventional epidemiology of human astroviruses. From 2010 to 2016, 232 stool samples from children under 5 years of age were screened using NGS and conventional RT-PCR followed by genetic analysis in order to investigate the genotypic diversity of classical human astrovirus (HAstV) circulating in Tocantins State, Brazil. HAstV was detected in 16 cases (6.9%). Seven specimens (43.7%; 7/16) were positive according RT-PCR and next-generation sequencing (NGS) to investigate the molecular to both NGS and RT-PCR. NGS and RT-PCR individually revealed six (37.5%; 6/16) and three (18.8%; 3/16) additional positive samples, respectively. Sequencing of the HAstV-positive samples revealed HAstV-1a (9/16), HAstV-4c (3/16), and HAstV-5c (4/16) lineages.

Bibliographic Details

Luchs, Adriana; Tardy, Kaelan; Tahmasebi, Roozbeh; Morillo, Simone Guadagnucci; Milagres, Flavio Augusto de Pádua; Morais, Vanessa Dos Santos; Brustulin, Rafael; Teles, Maria da Aparecida Rodrigues; de Azevedo, Lais Sampaio; de Souza, Ellen Viana; Medeiros, Roberta Salzone; de Souza, Yasmin França Viana Pires; Araújo, Emerson Luiz Lima; Witkin, Steven S; Deng, Xutao; Delwart, Eric; Sabino, Ester Cerdeira; Leal, Elcio; da Costa, Antonio Charlys

Springer Science and Business Media LLC

Immunology and Microbiology

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