Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature
Applied Magnetic Resonance, ISSN: 0937-9347, Vol: 44, Issue: 6, Page: 649-670
2013
- 26Citations
- 36Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations26
- Citation Indexes26
- 26
- CrossRef20
- Captures36
- Readers36
- 36
Article Description
In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron-electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for K-band measurements is 14-17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to K-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. © 2012 Springer-Verlag Wien.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84877599163&origin=inward; http://dx.doi.org/10.1007/s00723-012-0434-6; http://www.ncbi.nlm.nih.gov/pubmed/23687407; http://link.springer.com/10.1007/s00723-012-0434-6; https://dx.doi.org/10.1007/s00723-012-0434-6; https://link.springer.com/article/10.1007/s00723-012-0434-6
Springer Science and Business Media LLC
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