Quercetin Enhances Human α7 Nicotinic Acetylcholine Receptor-Mediated Ion Current through Interactions with Ca 2+ Binding Sites
Molecules and Cells, ISSN: 1016-8478, Vol: 30, Issue: 3, Page: 245-254
2010
- 24Citations
- 14Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations24
- Citation Indexes24
- 24
- CrossRef22
- Captures14
- Readers14
- 14
Article Description
The flavonoid quercetin is a low molecular weight substance found in fruits and vegetables. Aside from its anti-oxidative effect, quercetin, like other flavonoids, has a wide range of neuropharmacological actions. The α7 nicotinic acetylcholine receptor (α7 nAChR) has a Ca 2+ -binding site, is highly permeable to the Ca 2+ ion, and plays important roles in Ca 2+ -related normal brain functions. Dysfunctions of α7 nAChR are associated with a variety of neuro-logical disorders. In the present study, we investigated the effects of quercetin on the ACh-induced inward peak current ( IACh ) in Xenopus oocytes that heterologously express human α7 nAChR. IACh was measured with the two-electrode voltage clamp technique. In oocytes injected with α7 nAChR cRNA, the effects of the co-application of quercetin on IACh were concentration-dependent and reversible. The ED 50 was 36.1 + 6.1 μM. Quercetin-mediated enhancement of IACh caused more potentiation when quercetin was pre-applied. The degree of IACh potentiation by quercetin pre-application was time-dependent and saturated after 1 min. Quercetin-mediated IACh enhancement was not affected by ACh concentration and was voltage-independent. However, quercetin-mediated IACh enhancement was dependent on extracellular Ca 2+ concentrations and was specific to the Ca 2+ ion, since the removal of extracellular Ca 2+ or the addition of Ba 2+ instead of Ca 2+ greatly diminished quercetin enhancement of IACh. The mutation of Glu195 to Gln195, in the Ca 2+ -binding site, almost completely diminished quercetin mediated IACh enhancement. These results indicate that quercetin-mediated IACh enhancement human α7 nAChR heterologously expressed in Xenopus oocytes could be achieved through interactions with the Ca 2+ -binding site of the receptor.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S1016847823115068; http://dx.doi.org/10.1007/s10059-010-0117-9; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78049318379&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/20803082; https://linkinghub.elsevier.com/retrieve/pii/S1016847823115068; https://dx.doi.org/10.1007/s10059-010-0117-9; http://www.springerlink.com/index/10.1007/s10059-010-0117-9; http://www.springerlink.com/index/pdf/10.1007/s10059-010-0117-9
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know