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Fabrication of Highly Anisotropic and Interconnected Porous Scaffolds to Promote Preosteoblast Proliferation for Bone Tissue Engineering

Chinese Journal of Polymer Science (English Edition), ISSN: 1439-6203, Vol: 39, Issue: 9, Page: 1191-1199
2021
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Article Description

Mimicking the complex structure of natural bone remains a challenge for bone tissue scaffolds. In this study, a novel processing strategy was developed to prepare the bone-like scaffolds that are featured by highly oriented and fully interconnected pores. This type of biomimetic scaffolds was evolved from solid phase stretching of immiscible polycaprolactone (PCL)/poly(ethylene oxide) (PEO) blends with co-continuous structure and the pore morphology was inherited from selective extraction of water soluble PEO phase. The pore anisotropy was readily tuned by varying the stretching strain without loss of interconnectivity. Significant promotion in preosteoblast proliferation, alkaline phosphatase activity and osteogenic gene expression was observed in the oriented porous scaffolds compared to the isotropic porous counterpart. The oriented architecture provided a topographical cue for aligned growth of preosteoblasts, which activated the Wnt/β-catenin signaling pathway. The proposed strategy enriches the toolbox for the scaffold design and fabrication for bone tissue engineering.

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