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Parameterization of hyperpolarized C-bicarbonate-dissolution dynamic nuclear polarization

Magnetic Resonance Materials in Physics, Biology and Medicine, ISSN: 0968-5243, Vol: 28, Issue: 6, Page: 591-598
2015
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Article Description

Objective: C metabolic MRI using hyperpolarized C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated. Materials and methods: Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral–spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis. Results: An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19–21 % is presented; T and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated. Conclusion: An optimized bicarbonate preparation and experimental procedure provided improved T and SNR values, allowing in vitro and in vivo applications.

Bibliographic Details

Scholz, David Johannes; Otto, Angela M; Hintermair, Josef; Schilling, Franz; Frank, Annette; Köllisch, Ulrich; Janich, Martin A; Schulte, Rolf F; Schwaiger, Markus; Haase, Axel; Menzel, Marion I

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology; Health Professions; Medicine

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