Experimental study on the determination and degradation of pyoluteorin in soil via CE with Soxhlet's extraction and field-amplified sample stacking

A sensitive analytical method based on capillary electrophoresis with field-amplified sample stacking, Soxhlet's extraction and ultrasonic extraction for studying the degradation of trace amounts of pyoluteorin (Plt, a broad-spectrum fungicide produced by fluorescent Pseudomonas) in soil samples was first developed. The limits of detection and the limits of quantification for Plt of the developed method were 0.107 and 0.36 μg mL, respectively. The recoveries of Plt at three spiking concentrations of 500, 200, and 50 μg kg were 94.18, 79, and 78.25%, respectively. In this communication, we successfully performed the investigation on the dynamic residue of Plt during its decomposition in agriculture soil using the method developed. The calculated t values for Plt residue in near-surface and rhizosphere soil were 42.26 and 32.84 h, respectively. The results show that Plt has good environmental compatibility. © 2011 Springer-Verlag.