HPLC–DAD Method for the Quantification of Carbamazepine, Oxcarbazepine and their Active Metabolites in HepaRG Cell Culture Samples
Chromatographia, ISSN: 1612-1112, Vol: 79, Issue: 9-10, Page: 581-590
2016
- 8Citations
- 24Captures
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Article Description
A new, sensitive and fast high-performance liquid chromatography–diode-array detection assay is herein reported, for the first time, to simultaneously quantify carbamazepine (CBZ), oxcarbazepine (OXC), and the active metabolites carbamazepine-10,11-epoxide (CBZ-E) and licarbazepine (LIC) in HepaRG cell culture medium samples. Chromatographic separation of analytes (CBZ, CBZ-E, OXC, LIC) and internal standard (IS) was achieved in less than 15 min on a C-column, at 35 °C, using a mobile phase composed of water/methanol/acetonitrile (69:25:6 v/v/v) pumped at 1 mL min. The analytes and IS were detected at 215 nm. The method proved to be selective, accurate (bias ± 14.6 %), precise (coefficient of variation ≤13.1 %) and linear (r ≥ 0.9901) over the concentration ranges of 0.1–15 μg mL for CBZ; 0.1–5 μg mL for CBZ-E and OXC; and 0.1–40 μg mL for LIC. Furthermore, the absolute recovery of the analytes ranged from 64.5 to 96.9 % and their stability was demonstrated in the studied conditions. This validated HPLC assay will be a suitable tool to support future in vitro metabolism profiling, drug interaction and other pharmacokinetic-based studies in HepaRG cells involving these antiepileptic drugs (CBZ and OXC) and their main metabolites.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84961208349&origin=inward; http://dx.doi.org/10.1007/s10337-016-3063-7; http://link.springer.com/10.1007/s10337-016-3063-7; http://link.springer.com/content/pdf/10.1007/s10337-016-3063-7; http://link.springer.com/content/pdf/10.1007/s10337-016-3063-7.pdf; http://link.springer.com/article/10.1007/s10337-016-3063-7/fulltext.html; https://dx.doi.org/10.1007/s10337-016-3063-7; https://link.springer.com/article/10.1007/s10337-016-3063-7
Springer Science and Business Media LLC
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