Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan

A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated as NCCP-1340, was isolated from a hot water spring, Tatta Pani, Pakistan. Cells of strain NCCP-1340were observed to be aerobic, rod shaped, catalase and urease positive but HS production and oxidase negative. Growth was observed at pH 6.0–8.0 (optimum pH 7.0) and at 20–40 °C (optimum 37 °C). The strain could tolerate 0–8 % NaCl (optimum 2 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NCCP-1340belongs to the genus Nocardioides and is closely related to Nocardioides iriomotensis JCM 17985(96.8 %), Nocardioides daedukensis KCTC 19601(96.6 %), Nocardioides jensenii KCTC 9134(96.1 %) and Nocardioides daejeonensis KCTC 19772(96.1 %). The DNA–DNA relatedness values of strain NCCP-1340with N. iriomotensis JCM 17985, N. daedukensis KCTC 19601and N. jensenii KCTC 9134were found to be less than 53 %. The DNA G+C content of strain NCCP-1340was determined to be 71.8 mol %. The affiliation of strain NCCP-1340to the genus Nocardioides was further supported by chemotaxonomic data which showed the presence of MK-8(H) as major menaquinone system; iso-C, C, C10-methyl, iso-Cand C as major cellular fatty acids; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and unidentified glycolipids and polar lipids in the polar lipids profile. The cell wall peptidoglycan contained LL-diaminopimelic acid as the diagnostic amino acid. On the basis of physiological and biochemical characteristics and the phylogenetic analyses, strain NCCP-1340can be distinguished from the closely related taxa and thus represents a novel species of the genus Nocardioides, for which the name Nocardioides pakistanensis sp. nov. is proposed with the type strain NCCP-1340(= DSM 29942 = JCM 30630).