Siccirubricoccus soli sp. nov., a novel bacterial species isolated from Jiaozi Mountain soil

An isolate of Gram-stain-negative and strictly aerobic bacterium, designated KC 17139, was isolated from Jiaozi Mountain sample in Yunnan, China. Cells were non-motile cocci to oval, catalase-positive and oxidase-positive. Growth occurred at 0–7% NaCl (w/v; optimum, 0%), pH 6.0–8.0 (optimum, pH 7.0) and 15–45 °C (optimum, 28–37 °C). The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC) and four unidentified aminolipids (UALs). Strain KC 17139 contained summed feature 8 (comprising Cω7c and/or Cω6c), C 2OH and C as major cellular fatty acids (> 5%) and ubiquinone-10 as the sole isoprenoid quinone. The 16S rRNA gene sequence analysis indicated that strain KC 17139 shared highest similarities with Siccirubricoccus phaeus 1–3 (96.7%) and Siccirubricoccus deserti SYSU D8009 (95.0%). Strain KC 17139 clustered with the two Siccirubricoccus type strains, but formed a separate branch in both 16S rRNA gene and genome-scale phylogenetic dendrograms. The genomic DNA G + C content of strain KC 17139 was 71.2%. Genomic comparisons between strain KC 17139 and its close relatives showed the highest digital DNA–DNA hybridisation to S. phaeus (35.5%), highest average nucleotide identity to S. phaeus (88.2%), indicating that strain KC 17139 represents a novel species. On the basis of results of phenotypic, chemotaxonomic and molecular analysis, we report a new bacterium strain KC 17139 belonged to genus Siccirubricoccus, for which the name Siccirubricoccus soli sp. nov. is proposed. The type strain is KC 17139 (= CGMCC 1.18756 = JCM 35132).