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Synthesis and characterization of a novel near-infrared fluorescent probe for applications in imaging A549 cells

Biotechnology Letters, ISSN: 1573-6776, Vol: 38, Issue: 11, Page: 1851-1856
2016
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Article Description

Objective: To design and synthesize a novel near-infrared (NIR) fluorescent probe based on indocyanine Green (ICG), that can be applied in imaging living cells. Results: A highly fluorescent novel NIR fluorescent probe (IR-793) was synthesized in two steps. IR-793 had better fluorescence and optical stability than ICG. In addition, no obvious cytotoxicity effect of IR-793 was observed and cell viability was above 75% at the maximum concentration (120 nM). IR-793 also exhibited good performance in imaging living A549 cells. Conclusion: IR-793, a novel NIR fluorescent probe that is stable, low-cost, highly fluorescent and low cytotoxicity, has been designed and synthesized for imaging living cells.

Bibliographic Details

Xing, Jing; Zhou, Gaoxin; Sun, Chunlong; Zhang, Huanqing; Chen, Bo; Zong, Xi; Cai, Jin; Ji, Min

Springer Nature

Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Immunology and Microbiology

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