Functional roles of TGF-β in intestinal epithelial cells through smad-dependent and non-smad pathways
Digestive Diseases and Sciences, ISSN: 0163-2116, Vol: 58, Issue: 5, Page: 1207-1217
2013
- 21Citations
- 26Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations21
- Citation Indexes21
- 21
- CrossRef17
- Captures26
- Readers26
- 26
Article Description
Background and Aims: Transforming growth factor-β (TGF-β) is one of the growth factors expressed in the gut, and has been shown to play an important role in intestinal mucosal healing. We investigated the effects of TGF-β on the cellular functions of intestinal epithelial cells, and also evaluated its signaling pathways in these cells. Methods: We used the rat IEC-6 intestinal epithelial cell line for these studies. The expression of TGF-β/Smad signaling molecules was examined. We evaluated the effect of TGF-β on the proliferation and differentiation by the BrdU incorporation assay and real-time PCR. We manipulated the expression levels of Smad2 and Smad3 using an adenovirus system and small interfering RNA to examine the signaling pathways. The expression of Smad2 and Smad3 along the crypt-villus axis was also examined in the murine intestine. Results: IEC-6 cells produced TGF-β and expressed functional TGF-β/Smad signaling molecules. The addition of TGF-β in the culture medium suppressed the proliferation and increased the expression of a differentiation marker of enterocytes, in a dose-dependent manner. The adenovirus-mediated and small interfering RNA-mediated studies clearly showed that the growth inhibitory effect and the promotion of differentiation were exerted through a Smad3-dependent and a Smad2-dependent pathway, respectively. IEC-6 cells exhibited upregulated expression of an inhibitory Smad (Smad7) as a form of negative feedback via a non-Smad pathway. Smad2 was predominantly expressed in villi, and Smad3 in crypts. Conclusions: TGF-β regulates the cellular functions of intestinal epithelial cells through both Smad-dependent and non-Smad pathways. © 2013 Springer Science+Business Media New York.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84878697506&origin=inward; http://dx.doi.org/10.1007/s10620-012-2515-7; http://www.ncbi.nlm.nih.gov/pubmed/23306843; http://link.springer.com/10.1007/s10620-012-2515-7; https://dx.doi.org/10.1007/s10620-012-2515-7; https://link.springer.com/article/10.1007/s10620-012-2515-7
Springer Science and Business Media LLC
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