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Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey

Journal of Applied Spectroscopy, ISSN: 0021-9037, Vol: 82, Issue: 5, Page: 845-848
2015
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Article Description

Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denaturation of BSA, which otherwise shows a two-step, three-state transition, using intrinsic fluorescence of the protein as the probe failed to produce any result in the presence of 5% (w/v) honey. Thus, intrinsic fluorescence cannot be used as a spectral probe for protein denaturation studies in the presence of honey.

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