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The VEGF +405 G>C 5' untranslated region polymorphism and risk of PCOS: a study in the South Indian Women

Journal of Assisted Reproduction and Genetics, ISSN: 1573-7330, Vol: 31, Issue: 10, Page: 1383-1389
2014
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Article Description

Purpose: The aim of this study was to investigate the association between two common single nucleotide polymorphisms (SNPs) in the vascular endothelial growth factor (VEGF) gene (−460C/T and +405G/C) and polycystic ovary syndrome (PCOS) risk in south Indian women. Methods: This study involves clinically confirmed PCOS patients (n = 126) and non-PCOS controls (n = 130) of south Indian origin (Dravidian linguistic group). Genotyping of the VEGF gene −460C/T and +405G/C SNPs were performed by PCR and sequencing analysis. Haplotype frequencies for multiple loci and the standardized disequilibrium coefficient (D') for pairwise linkage disequilibrium (LD) were assessed by Haploview Software. Results: The frequencies of +405G/G genotype (P = 0.03) and +405G alleles (P = 0.006) were significantly higher in patients compared to controls. Whereas the genotype and allele frequencies of −460C/T SNP were not significantly different between patients and controls. In addition, LD analysis revealed no significant difference between patients and controls. Conclusion: Our findings suggest that the VEGF +405G/C polymorphism may constitute an inheritable risk factor for PCOS in south Indian women.

Bibliographic Details

Guruvaiah, Praveen; Govatati, Suresh; Reddy, Tumu Venkat; Lomada, Dakshayani; Deenadayal, Mamata; Shivaji, Sisinthy; Bhanoori, Manjula

Springer Science and Business Media LLC

Medicine; Biochemistry, Genetics and Molecular Biology

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