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mCICR is required for AsO-induced permeability transition pore opening and cytochrome c release from mitochondria

Molecular and Cellular Biochemistry, ISSN: 0300-8177, Vol: 277, Issue: 1-2, Page: 33-42
2005
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Article Description

The permeability transition pore (PTP) is central for apoptosis by acting as a good candidate pathway for the release of Cyt. c and apoptosis induction factors (AIF). Arsenite induces apoptosis via a direct effect on PTP. To characterize the exact mechanism for arsenite induces PTP opening, the effect of Ca on As O-induced PTP opening, the relationship between AsO-induced PTP opening and Cyt. c release from mitochondria and calcium-induced calcium release from mitochondria (mCICR), and the effects of AsO on Ca-induced PTP opening were studied. The results showed AsO induces Cyt. c release by triggering PTP opening. Ca is necessary for AsO -induced PTP opening. AsO-induced PTP opening and Cyt. c release depends on mCICR. AsO promotes PTP opening by lowering Ca-threshold. These results indicated AsO induce Cyt. c release from mitochondria by lowering Ca-threshold for PTP and triggering mCICR-dependent PTP opening. Suggesting that it is possible to control apoptosis by altering Ca threshold and mCICR to modulate PTP opening and Cyt. c release. © Springer Science + Business Media, Inc. 2005.

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