Stabilizing action of tetrahydrobiopterin on the activity of neuronal nitric oxide synthase
Neurophysiology, ISSN: 0090-2977, Vol: 44, Issue: 5, Page: 351-355
2012
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Article Description
We studied the effect of tetrahydrobiopterin (BH), one of the co-factors of NO synthase (NOS), on the process of inactivation of this enzyme in a cytoplasmic fraction obtained from rat cerebellar tissue. Tetrahydrobiopterin stimulated in a dose-dependent manner the activity of NOS measured according to accumulation of [H]-citrulline. In the course of reaction at 37°C without the addition of BH, the enzymatic activity rapidly decreased; in the presence of 10 μM BH, the rate of inactivation of NOS was noticeably smaller. Preincubation (40 min long) under the same conditions but in the absence of substrates (arginine and NADPH) and BH also led to a decrease in the enzymatic activity. Preincubation in the presence of 1 mM BH not only slowed down inactivation of NOS but also provided an appreciable increase in its activity, as compared with the variant without preincubation. This fact is indicative of the presence of a BH-free inactive form of the above-mentioned enzyme in the cytoplasmic fraction, which can be additionally activated. The obtained data show that BH stabilizes the activity of NOS due to a decrease in the rate of inactivation of this enzyme occurring independently of realization of the enzymatic reaction. © 2012 Springer Science+Business Media New York.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84872222160&origin=inward; http://dx.doi.org/10.1007/s11062-012-9305-x; http://link.springer.com/10.1007/s11062-012-9305-x; http://www.springerlink.com/index/10.1007/s11062-012-9305-x; http://www.springerlink.com/index/pdf/10.1007/s11062-012-9305-x; https://dx.doi.org/10.1007/s11062-012-9305-x; https://link.springer.com/article/10.1007/s11062-012-9305-x
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