Expression of multidrug resistance proteins (Mrps) in astrocytes of the mouse brain: A single cell RT-PCR study
Neurochemical Research, ISSN: 0364-3190, Vol: 30, Issue: 10, Page: 1237-1244
2005
- 26Citations
- 19Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations26
- Citation Indexes26
- 26
- CrossRef17
- Captures19
- Readers19
- 19
Article Description
Multidrug resistance proteins (Mrps) are ATP-driven export pumps which mediate the export of organic anions such as glutathione conjugates and glucuronides from eukaryotic cells. Within the central nervous system astrocytes have important functions in metabolism and detoxification. In such processes Mrps play essential roles. To identify the Mrp repertoire of mouse brain and of astrocytes in particular, the expression of six mouse Mrps was investigated by reverse-transcription polymerase-chain reaction (RT-PCR). Using mouse brain mRNA as source, amplification products were obtained for Mrp1, Mrp3, Mrp4, Mrp5 and Mrp6. In contrast, mRNA of Mrp2 could not be detected in mouse brain. To investigate whether individual astrocytes express different Mrps in brain, single-cell RT-PCRs were performed from the cytosol harvested from single astrocytes in acutely isolated brain slices from cortex and cerebellum of TgN(GFAP-EGFP) mice. In these mice astrocytes can readily be identified by glial fibrillary acidic protein promoter-controlled green fluorescent protein expression. Investigation of individual cortical astrocytes and Bergmann glial cells revealed that these cells express Mrp1, Mrp4 and Mrp5 and that individual astrocytes can contain mRNA of one, two or three of these Mrps simultaneously. © 2005 Springer Science+Business Media, Inc.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=28844464795&origin=inward; http://dx.doi.org/10.1007/s11064-005-8795-y; http://www.ncbi.nlm.nih.gov/pubmed/16341585; http://link.springer.com/10.1007/s11064-005-8795-y; https://dx.doi.org/10.1007/s11064-005-8795-y; https://link.springer.com/article/10.1007/s11064-005-8795-y; http://www.springerlink.com/index/10.1007/s11064-005-8795-y; http://www.springerlink.com/index/pdf/10.1007/s11064-005-8795-y
Springer Science and Business Media LLC
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