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Isolation of Plant Gene space-Related sequence elements by high C+G patch (HCGP) filtration: Model Study on rice

Plant Molecular Biology Reporter, ISSN: 0735-9640, Vol: 27, Issue: 1, Page: 79-85
2009
  • 2
    Citations
  • 0
    Usage
  • 15
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    2
    • Citation Indexes
      2
  • Captures
    15

Article Description

For the isolation of gene space representative sequence elements, a new methodology-high C+G patch (HCGP) filtration-has been developed using rice as a model. The method is based on the fragmentation of the genomic DNA by methylation-sensitive HpaII and MspI restriction endonucleases having exclusively G/C base pair-containing recognition sites. These enzymes fragment the genome at high C+G content and hypomethylated regions. Cloning fragments spanning such regions in close vicinity (200-2,000 bp) revealed that about 60% of the clones represented gene space sequences resulting in twofold enrichment of these sequences, which is close to the theoretical maximum in rice. © Springer-Verlag 2008.

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