Isolation of Plant Gene space-Related sequence elements by high C+G patch (HCGP) filtration: Model Study on rice
Plant Molecular Biology Reporter, ISSN: 0735-9640, Vol: 27, Issue: 1, Page: 79-85
2009
- 2Citations
- 15Captures
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Article Description
For the isolation of gene space representative sequence elements, a new methodology-high C+G patch (HCGP) filtration-has been developed using rice as a model. The method is based on the fragmentation of the genomic DNA by methylation-sensitive HpaII and MspI restriction endonucleases having exclusively G/C base pair-containing recognition sites. These enzymes fragment the genome at high C+G content and hypomethylated regions. Cloning fragments spanning such regions in close vicinity (200-2,000 bp) revealed that about 60% of the clones represented gene space sequences resulting in twofold enrichment of these sequences, which is close to the theoretical maximum in rice. © Springer-Verlag 2008.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=70349993646&origin=inward; http://dx.doi.org/10.1007/s11105-008-0063-2; http://link.springer.com/10.1007/s11105-008-0063-2; http://link.springer.com/content/pdf/10.1007/s11105-008-0063-2; http://link.springer.com/content/pdf/10.1007/s11105-008-0063-2.pdf; http://link.springer.com/article/10.1007/s11105-008-0063-2/fulltext.html; https://dx.doi.org/10.1007/s11105-008-0063-2; https://link.springer.com/article/10.1007/s11105-008-0063-2; http://www.springerlink.com/index/10.1007/s11105-008-0063-2; http://www.springerlink.com/index/pdf/10.1007/s11105-008-0063-2
Springer Science and Business Media LLC
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