The creation of transgenic pigs expressing human proteins using BAC-derived, full-length genes and intracytoplasmic sperm injection-mediated gene transfer
Transgenic Research, ISSN: 0962-8819, Vol: 21, Issue: 3, Page: 605-618
2012
- 30Citations
- 13Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations30
- Citation Indexes29
- 29
- CrossRef18
- Policy Citations1
- Policy Citation1
- Captures13
- Readers13
- 13
Article Description
In most transgenic (Tg) animals created to date, a transgene consisting of the minimum promoter region linked to a cDNA has been used. However, transgenes on small plasmids are susceptible to the position effect, increasing the difficulty of controlling transgene expression. In this study, we attempted to create Tg pigs by intracytoplasmic sperm injection-mediated gene transfer (ICSI-MGT) using two large genomic transgenes derived from a bacterial artificial chromosome (BAC) containing the full genomic region encoding two human proteins, type I collagen and albumin. The production efficiencies (Tg piglets/live offspring) of type I collagen and albumin Tg pigs were 11.8% (6/51) and 18.2% (2/11), respectively. In all of the Tg pigs examined by real-time PCR analysis, tissue-specific expression of the transgene was confirmed (type I collagen: skin, tendon, vessels, genitalia; albumin: liver). The production of human proteins derived from BAC transgenes was also confirmed. Fluorescence in situ hybridization analysis indicated that the BAC transgenes transferred into porcine oocytes by ICSI-MGT were integrated into single or multiple sites on the host chromosomes. These data demonstrate that Tg pigs expressing human proteins in a tissue-specific manner can be created using a BAC transgenic construct and the ICSI-MGT method. © 2011 Springer Science+Business Media B.V.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84860889151&origin=inward; http://dx.doi.org/10.1007/s11248-011-9561-3; http://www.ncbi.nlm.nih.gov/pubmed/22038447; http://link.springer.com/10.1007/s11248-011-9561-3; http://www.springerlink.com/index/10.1007/s11248-011-9561-3; http://www.springerlink.com/index/pdf/10.1007/s11248-011-9561-3; https://dx.doi.org/10.1007/s11248-011-9561-3; https://link.springer.com/article/10.1007/s11248-011-9561-3
Springer Science and Business Media LLC
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