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Detection of the key enzyme of alginate biosynthesis in Vibrio sp. QY102

World Journal of Microbiology and Biotechnology, ISSN: 0959-3993, Vol: 24, Issue: 8, Page: 1613-1615
2008
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Article Description

Alginate is an important component of biofilms of many pathogens, but its presence in Vibrio has not been reported. The GDP-mannose dehydrogenase gene (algD), which is the kinetic control point in alginate biosynthesis, was cloned for the first time from Vibrio species using degenerated PCR and inverse PCR. Sequence analysis showed that algD was also localized in an alginate biosynthesis cluster, as it is in Pseudomonas aeruginosa. In addition, the existence of mannuronic acid, a component of alginate, was supported by the NMR spectrum of Vibrio sp. QY102 exopolysaccharide. © 2007 Springer Science+Business Media B.V.

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