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Chondrogenic differentiation of mouse embryonic stem cells promoted by mature chondrocytes

Science in China, Series C: Life Sciences, ISSN: 1006-9305, Vol: 51, Issue: 9, Page: 774-778
2008
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Article Description

In order to direct embryonic stem (ES) cells to differentiate into chondrocytes, a chondrogenic environment provided by mature chondrocytes was investigated. Flk-1 positive cells sorted from pre-differentiated mouse ES cells were mixed with adult porcine articular chondrocytes, seeded on biodegradable scaffolds, and then implanted subcutaneously into nude mice. The cell-scaffold complexes formed cartilage tissues after 4 weeks, which was demonstrated by histology and anti-type II collagen antibody staining. Positive staining of mouse Major Histocompatibility Complex class I molecules confirmed that part of the chondrocytes were derived from mouse ES cells. The current study established a new approach for directing ES cell differentiation. © 2008 Science in China Press and Springer-Verlag GmbH.

Bibliographic Details

Xie, Feng; Zhang, WenJie; Chen, FanFan; Zhou, GuangDong; Cui, Lei; Liu, Wei; Cao, YiLin

Springer Science and Business Media LLC

Energy; Mathematics; Biochemistry, Genetics and Molecular Biology; Environmental Science; Agricultural and Biological Sciences

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