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Integrin-interacting protein Kindlin-2 induces mammary tumors in transgenic mice

Science China Life Sciences, ISSN: 1674-7305, Vol: 62, Issue: 2, Page: 225-234
2019
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Article Description

Kindlin-2, an integrin-interacting protein, regulates breast cancer progression. However, currently, no animal model to study the role of Kindlin-2 in the carcinogenesis of mammary gland is available. We established a Kindlin-2 transgenic mouse model using a mammary gland-specific promoter, mammary tumor virus (MMTV) long terminal repeat (LTR). Kindlin-2 was overexpressed in the epithelial cells of the transgenic mice. The mammary gland ductal trees were found to grow faster in MMTV-Kindlin-2 transgenic mice than in control mice during puberty. Kindlin-2 promoted mammary gland growth as indicated by more numerous duct branches and larger lumens, and more alveoli were formed in the mammary glands during pregnancy under Kindlin-2 overexpression. Importantly, mammary gland-specific expression of Kindlin-2 induced tumor formation at the age of 55 weeks on average. Additionally, the levels of estrogen receptor and progesterone receptor were decreased, whereas human epidermal growth factor receptor 2 and β-catenin were upregulated in the Kindlin-2-induced mammary tumors. These findings demonstrated that Kindlin-2 induces mammary tumor formation via activation of the Wnt signaling pathway.

Bibliographic Details

Li, Bing; Chi, Xiaochun; Song, Jiagui; Tang, Yan; Du, Juan; He, Xiaokun; Sun, Xiaoran; Bi, Zhenwu; Wang, Yunling; Zhan, Jun; Zhang, Hongquan

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology; Environmental Science; Agricultural and Biological Sciences

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