Application of an ionic liquid-functionalized MgAl layered double hydroxide for the electrochemical myoglobin biosensor
Ionics, ISSN: 1862-0760, Vol: 20, Issue: 10, Page: 1471-1479
2014
- 11Citations
- 17Captures
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Article Description
In this paper, an ionic liquid 1-carboxyl-methyl-3-methylimidazolium tetrafluoroborate (CMMIMBF)-functionalized MgAl layered double hydroxide (LDH) was synthesized and further used for the immobilization of myoglobin (Mb) on the surface of a carbon ionic liquid electrode to get a new electrochemical biosensor. Ultraviolet–visible and Fourier transform–infrared spectroscopies confirmed that Mb in the CMMIMBF-LDH remained its native secondary structure, which was attributed to the biocompatibility of the materials used. On the cyclic voltammograms, a pair of well-defined redox peaks appeared, indicating that direct electron transfer of Mb was realized in the modified electrode. The formal peak potential was calculated as −0.209 V (vs. SCE), which was the typical characteristics of the Mb heme Fe(III)/Fe(II) redox couples. The fabricated Mb sensor exhibited good electrocatalytic activity to the reduction of trichloroacetic acid in the range from 1.0 to 17.0 mmol L with the detection limit as 0.344 mmol L (3σ), and the apparent Michaelis–Menten constant was calculated as 13.5 mmol L. Thus, the ionic liquid-functionalized LDH exhibited the potential application in the electrochemical sensor for redox proteins.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84920254598&origin=inward; http://dx.doi.org/10.1007/s11581-014-1088-1; http://link.springer.com/10.1007/s11581-014-1088-1; http://link.springer.com/content/pdf/10.1007/s11581-014-1088-1; http://link.springer.com/content/pdf/10.1007/s11581-014-1088-1.pdf; http://link.springer.com/article/10.1007/s11581-014-1088-1/fulltext.html; https://dx.doi.org/10.1007/s11581-014-1088-1; https://link.springer.com/article/10.1007/s11581-014-1088-1
Springer Science and Business Media LLC
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