Isolation of murine aortic endothelial cells in culture and the effects of sex steroids on their growth
In Vitro Cellular and Developmental Biology - Animal, ISSN: 1071-2690, Vol: 39, Issue: 3-4, Page: 140-145
2003
- 16Citations
- 23Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations16
- Citation Indexes16
- 16
- CrossRef14
- Captures23
- Readers23
- 23
Article Description
The lack of commercially available primary murine endothelial cells prompted us to isolate and cultivate this cell type. We report here the effect of sex steroids on the in vitro growth of murine aortic endothelial cells. Murine aortic endothelial cells were isolated by a combination of explant outgrowth from aortic rings and enzymatic digestion. The endothelial nature of the cells was verified by uptake of acylated low-density lipoprotein and positive staining for CD-31. Murine aortic endothelial cell growth is stimulated by physiological concentrations of estrogen. Progesterone, when given simultaneously with estrogen, inhibited the stimulatory growth effect of estrogen. Murine aortic endothelial cells grown in vitro continue to express messenger ribonucleic acid for proteins related to endothelial growth. These include vascular endothelial growth factor, its receptors Flt-1 and Flk-1, and the angiogenesis-associated transcription factor, Ets-1.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0141455083&origin=inward; http://dx.doi.org/10.1007/s11626-003-0008-x; http://www.ncbi.nlm.nih.gov/pubmed/14505433; http://link.springer.com/10.1007/s11626-003-0008-x; http://www.springerlink.com/index/10.1007/s11626-003-0008-x; http://www.springerlink.com/index/pdf/10.1007/s11626-003-0008-x; https://dx.doi.org/10.1007/s11626-003-0008-x; https://link.springer.com/article/10.1007/s11626-003-0008-x
Springer Science and Business Media LLC
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