PlumX Metrics
Embed PlumX Metrics

Micropropagation of cocoyam (Xanthosoma sagittifolium L. Schott) in temporary immersion bioreactor

Plant Biotechnology Reports, ISSN: 1863-5466, Vol: 7, Issue: 3, Page: 383-390
2013
  • 18
    Citations
  • 0
    Usage
  • 34
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    18
    • Citation Indexes
      18
  • Captures
    34

Article Description

This study aims at establishing a temporary immersion technique for large-scale propagation of cocoyam (Xanthosoma sagittifolium). Sucrose was experimented with as a determinant factor for shoot multiplication in this culture system. The highest proliferation rate (68 ± 7) occurred with 20 g l sucrose in the culture medium. This concentration appeared to be the optimal amount due to its promoting effect on plantlet development. The acclimatized plantlets showed a continuous effect of sucrose treatment during ex vitro growth, especially in low sucrose concentration. This fact is evidenced by the low survival rate (0.13 ± 0.12) and the poor chlorophyll content (1.180 ± 0.076 mg g) recorded on plantlets derived from 15 g l of sucrose. The treatment with 60 g l of sucrose prior to acclimatization was efficient for roots induction and elongation. The analysis of pH revealed a fluctuation from one subculture to another, with an overall pH decrease under all treatments tested and, thus, indicates that plants release proton during growth. This feature had an impact on in vitro plantlets growth. The potentiality of the temporary immersion technique to foster the production of Xanthosoma sagittifolium is discussed. © 2013 Korean Society for Plant Biotechnology and Springer Japan.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know