Il17A autoantibody induced by recombinant protein Ag85A-Il17A
Applied Biochemistry and Biotechnology, ISSN: 0273-2289, Vol: 169, Issue: 2, Page: 502-510
2013
- 3Citations
- 11Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations3
- Citation Indexes3
- CrossRef3
- Captures11
- Readers11
- 11
Article Description
IL17A is a widely studied cytokine which plays an important role in autoimmune diseases as well as asthma. In this study, murine IL17a gene fragment was inserted into pET28a-Ag85A and it was expressed as a fusion protein named Ag85A-IL17A. Recombinant protein Ag85A-IL17A was expressed as an open reading frame of 1,287 bp, encoding a polypeptide of 429 amino acid residues with a predicted molecular mass of 50 kDa. It was expressed in Escherichia coli. BL21 and purified by metal affinity chromatography using a nickel-nitrilotriacetic acid column. Bioinformatics revealed that Ag85A-IL17A contained B cell epitopes of IL17A. Purified proteins Ag85A-IL17A and Ag85A were injected with adjuvants into mice then antibody responses in sera were measured by enzyme-linked immunosorbent assay and western blot assay. Experiments showed that purified protein Ag85-IL17A could bind to a standard IL17A antibody and could induce IL17A autoantibody in mice. © 2012 Springer Science+Business Media New York.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84873089601&origin=inward; http://dx.doi.org/10.1007/s12010-012-9994-z; http://www.ncbi.nlm.nih.gov/pubmed/23239413; http://link.springer.com/10.1007/s12010-012-9994-z; https://dx.doi.org/10.1007/s12010-012-9994-z; https://link.springer.com/article/10.1007/s12010-012-9994-z
Springer Science and Business Media LLC
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