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Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices

Food Analytical Methods, ISSN: 1936-976X, Vol: 4, Issue: 1, Page: 108-115
2011
  • 22
    Citations
  • 0
    Usage
  • 27
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    22
    • Citation Indexes
      22
  • Captures
    27

Article Description

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) as a new valuable technique has been used for qualitative screening of anthocyanins in wine and grape samples from Vranec and Merlot variety. For that purpose, different MALDI matrices have been tested, such as α-cyano-4-hydroxycinnamic acid, sinapic acid, 2,5-dihydroxybenzoic acid (2,5-DHB), and C fullerene. It was observed that 2,5-DHB was superior with respect to all the matrices used in the study. Furthermore, fullerene was, for the first time, tested and used as a possible matrix for MALDI-TOF-MS identification of anthocyanins in wine and grape samples. The results obtained showed identification of aglycone forms of anthocyanins only, since the higher laser energy applied for ionization of the fullerene molecules led to great deal of fragmentation of the sugar moiety from glucosides, acetylglucoside, and p-coumaroylglucoside groups from the corresponding anthocyanin molecules. Additionally, liquid chromatography/electrospray ionization mass spectrometry has also been applied for anthocyanin characterization. © 2010 Springer Science+Business Media, LLC.

Bibliographic Details

Violeta Ivanova; Ágnes Dörnyei; Marina Stefova; Trajće Stafilov; Borimir Vojnoski; Ferenc Kilár; László Márk

Springer Science and Business Media LLC

Chemistry; Agricultural and Biological Sciences; Immunology and Microbiology; Engineering; Social Sciences

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