Development of Sandwich ELISA and Immunochromatographic Strip for the Detection of Peanut Allergen Ara h 2
Food Analytical Methods, ISSN: 1936-976X, Vol: 8, Issue: 10, Page: 2605-2611
2015
- 35Citations
- 19Captures
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Article Description
In order to develop highly sensitive immunoassays for detecting the major peanut allergen, Ara h 2, we prepared six monoclonal antibodies (mAb) with high affinity to Ara h 2. Using mAb 5 as a capture antibody and HRP-labeled mAb 1 as a detection antibody, a sandwich enzyme-linked immunoassay (ELISA) was established with a limit of detection (LOD) of 0.02 ng/mL. An immunochromatographic strip based on these two mAbs was also established with a LOD of 1 ng/mL. Both tests showed high specificity in cross-reactivity experiments. Sample analyses indicated that these two immunoassays were feasible, accurate, and highly sensitive for measuring allergen Ara h 2 of food.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84942550577&origin=inward; http://dx.doi.org/10.1007/s12161-015-0163-1; http://link.springer.com/10.1007/s12161-015-0163-1; http://link.springer.com/content/pdf/10.1007/s12161-015-0163-1; http://link.springer.com/content/pdf/10.1007/s12161-015-0163-1.pdf; http://link.springer.com/article/10.1007/s12161-015-0163-1/fulltext.html; https://dx.doi.org/10.1007/s12161-015-0163-1; https://link.springer.com/article/10.1007/s12161-015-0163-1
Springer Science and Business Media LLC
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