On-line specific growth rate control for improving reduced glutathione production in Saccharomyces cerevisiae
Biotechnology and Bioprocess Engineering, ISSN: 1976-3816, Vol: 20, Issue: 5, Page: 887-893
2015
- 15Citations
- 23Captures
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Article Description
Reduced glutathione (GSH), the abundant bioactive tripeptide in most living cells, is widely used in pharmaceutical, food, and cosmetic industries. Specific growth rate (μ) is a key physiological parameter for GSH high-cell-density cultivation using microbial cell factories. Here, based on a biomass probe, an on-line μ feedback control was developed to regulate glucose feeding rate during the fed-batch phase for overproducing GSH in Saccharomyces cerevisiae. Compared with real-time μ controlled at 0.15/h, μ controlled at 0.2/h achieved yeast dry weight (120 g/L), GSH concentration (1.5 g/L), and intracellular GSH content (1.25%), which improved by 9, 150, and 129.1%, respectively. To our knowledge, this is the first report about on-line μ feedback control for GSH production. On-line μ control led to 59.38 mg/L/h of GSH productivity and 3.52 mg/g of GSH yield on glucose, which improved by 107.6 and 7.2%, respectively, in comparison with those of traditional ethanol feedback control (maintaining ethanol concentration at 1%). Taken together, the on-line μ feedback control is a promising method as an efficient alternative to conventional feed control techniques presently practiced in the GSH industry, and has the potential for the production of other valuable chemicals.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84947921514&origin=inward; http://dx.doi.org/10.1007/s12257-015-0018-z; http://link.springer.com/10.1007/s12257-015-0018-z; http://link.springer.com/content/pdf/10.1007/s12257-015-0018-z; http://link.springer.com/content/pdf/10.1007/s12257-015-0018-z.pdf; http://link.springer.com/article/10.1007/s12257-015-0018-z/fulltext.html; https://dx.doi.org/10.1007/s12257-015-0018-z; https://link.springer.com/article/10.1007/s12257-015-0018-z
Springer Science and Business Media LLC
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