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Safety Assessment of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA Determined in Trials with Wistar Rats.

Probiotics and antimicrobial proteins, ISSN: 1867-1306, Vol: 1, Issue: 1, Page: 15-23
2009
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Article Description

Colonization of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA in the gastro-intestinal tract was determined by using Wistar rats as model. The strains were administered through intragastric gavage over 14 days. FISH with strain-specific oligonucleotide probes indicated that Lact. plantarum 423 adhered to the surfaces of the ileum and the cecum. Enterococcus mundtii ST4SA, on the other hand, adhered to the surfaces of the cecum and colon. Results obtained by DGGE have shown that strains 423 and ST4SA excluded Enterobacteriaceae, but not lactic acid bacteria, from the cecum and colon. No signs of perforation of epithelial cells by strains 423 and ST4SA were detected. The spleen and liver appeared healthy and blood counts were normal, suggesting that the strains are not pathogenic. Both strains produce antimicrobial peptides active against a number of pathogens and may be considered as probiotics.

Bibliographic Details

Ramiah, K; Ten Doeschate, K; Smith, Rob; Dicks, Leon M T

Springer Science and Business Media LLC

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