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Development and characterization of 135 SNP markers in chiton Acanthochitona rubrolineatus

Conservation Genetics Resources, ISSN: 1877-7260, Vol: 14, Issue: 2, Page: 155-161
2022
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Article Description

Acanthochitona rubrolineatus (Lischke, 1873) (Polyplacophora, Neoloricata, Cryptoplacidae) is an important species widely distributed in the middle and low tide zone of coastal intertidal zone along the China Sea coast. In recent years, the serious pollution of seawater has caused a sharp decline in the number of wild populations of A. rubrolineatus. Lacking of effective molecular markers limits the effective protection and management of this species. Studies for the isolation and characterization of 135 A. rubrolineatus SNP markers were carried out. The frequency of minor allele ranges from 0.0125 to 0.5000. The observed heterozygosity and expected heterozygosity are 0.0000-0.9750, 0.0731–0.6696, respectively. The inbreeding value varies from − 0.2902 to 0.9966. Among them, there are 27 sites markablely differently from Hardy-Weinberg equilibrium (P < 0.05). The study of the polymorphic SNPs will provide a therotical basis for further analysis of population genetic analysis on A. rubrolineatus.

Bibliographic Details

Jirui Zhang; Yunqi Song; Chenghong Wang; Shukui Zhang; Zilin Jiang; Yan Ni; Ying Qu; Donghui Sun; Zhikai Xing; Sen Wu; Jiangyong Qu; Xumin Wang; Lijun Wang

Springer Science and Business Media LLC

Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology

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