Universal direct PCR amplification system: a time- and cost-effective tool for high-throughput applications
3 Biotech, ISSN: 2190-5738, Vol: 7, Issue: 4, Page: 246
2017
- 24Citations
- 81Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations24
- Citation Indexes24
- 24
- Captures81
- Readers81
- 81
Article Description
Taking into account the limits of current genotyping methodologies, we have established a versatile direct PCR method on intact microtissue samples without prior DNA isolation. A simple and standard protocol was developed and validated on a wide range of living organisms including bacterial and fungal strains, plant species and human samples. This allows reliable amplification of target genomic DNA fragment directly from source material using minimal amount of tissue which makes DNA purification irrelevant for a number of biological applications. The direct PCR technique established here represents an excellent alternative to traditional amplification methods used for real-time detection. Since this approach was efficiently and universally applied for high-throughput molecular screening, its implementation will offer new insights for several investigations in human health, biomedical diagnosis, plant biotechnology, as well as in applied environmental and food microbiology.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85024398614&origin=inward; http://dx.doi.org/10.1007/s13205-017-0890-7; http://www.ncbi.nlm.nih.gov/pubmed/28711981; http://link.springer.com/10.1007/s13205-017-0890-7; https://dx.doi.org/10.1007/s13205-017-0890-7; https://link.springer.com/article/10.1007/s13205-017-0890-7
Springer Nature
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