Optimization of pretreatment conditions for bamboo culm harvested at varying ages

For the production of bioethanol, bamboo is quickly becoming recognized as a promising feedstock among other bio-based sources with enormous promise. The age of the culm when it is harvested is the most important factor that determines whether or not bamboo may be used as a renewable supply. In this study, the responsive enhancements of Beema bamboo were investigated based on the age of the stem. Beema bamboo is a superior hybrid clone of Bambusa balcooa. For this investigation, the bamboo was harvested at various stages of its development and then subjected to a two-step pretreatment process (step 1 acid treatment and step 2 alkali treatment). The amount of sugar that can be recovered and the amount of ethanol that can be produced from the treatment have both been estimated using enzymatic hydrolysis of the pretreated bamboo. According to the findings of the study, different age groups of bamboo exhibited significantly different chemical compositions (p-value < 0.05). The two-step pretreatment and enzymatic hydrolysis of bamboo resulted in a 95% recovery of fermentable sugar and a theoretical maximum ethanol output of 92.2%. Two-year-old bamboo had the highest ethanol output (321L/Ton) across all age categories. Therefore, 2 years of age was the optimal harvesting age for maximizing sugar recovery for bioethanol production.