Evaluation of biomarkers in liver following Solanum melongena green calyx administration in diabetic rats

Background: Solanum melongena green calyx (SMGC) has antioxidant properties. Diabetes mellitus (DM) increases oxidative stress and causes cellular damages in liver. This study attempts to show the protective effects of SMGC against morphometric, inflammatory, oxidative, and apoptotic changes in liver following DM induction. Methods: For DM induction, the streptozotocin (60 mg/kg) was injected intraperitoneally. After the preparation of the SMGC extract, phytochemical content was analyzed. Sixty-four rats were categorized into 8 groups (n = 8); control, diabetic, SMGC, and diabetic + SMGC. SMGC administration was applied orally with doses of 100, 300, 500 mg/kg for 4 weeks. The assays of nitrite oxide, lipid peroxidation (LP), and Ferric Reducing Ability of Plasma (FRAP) were conducted for sample analysis. P53, Bcl2, and Bax genes expression, inflammatory cytokines, enzymes, and morphological features were measured. Apoptotic cell index, body weight, and levels of glucose and insulin were also analyzed. A one-way ANOVA test was used for statistical analysis. Result: According to the phytochemical analysis, the SMGC is rich in Tannins and Saponins. Antioxidant values, p53 and Bax genes expression, inflammatory cytokines, enzymes, body weight, serum glucose, and morphometrical features were increased significantly (except insulin and FRAP levels and Bcl2 gene expression which were decreased) in diabetic group compared to the control group (P < 0.05). Also, evaluated parameters were reduced significantly (except insulin and FRAP levels and Bcl2 gene expression which were increased) in SMGC and diabetic + SMGC groups in comparison with the diabetic group (P < 0.05). Conclusion: These findings revealed that the SMGC attenuates blood glucose levels in diabetic animals and also eliminates destructive effects of DM on liver through antioxidant features.