Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection
Chemical Research in Chinese Universities, ISSN: 2210-3171, Vol: 38, Issue: 2, Page: 529-534
2022
- 18Citations
- 6Captures
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Article Description
In this study, palladium nanoparticles loaded graphdiyne oxide (Pd/GDYO) nanocomposite were fabricated by in-situ reduction of palladium chloride in the dispersion of GDYO, and characterized by Raman spectra, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The synthesized Pd/GDYO was first found to have catalytic activities similar to those of the peroxidase enzyme, which can catalyze the oxidation of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine(TMB) in the presence of hydrogen peroxide(HO). Steady-state kinetic studies showed that the catalytic reaction of Pd/GDYO follows a ping-pong mechanism, and Pd/GDYO has a stronger activity to TMB with a Michaelis constant(K) value of 5.32×10 mmol/L. Based on the shielding effect of glutathione(GSH) on the Pd/GDYO-HO-TMB reaction system, a colorimetric detection method for GSH was developed with a wide linear range from 0.1 μmol/L to 40 μmol/L and a limit of detection of 0.1 μmol/L. The method was successfully applied for fast and accurate detection of GSH in injection powder drugs. It was expected that this peroxidase-like Pd/GDYO nanocomposite would have wide applications in the fields of biomedicine and environmental chemistry.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85105367162&origin=inward; http://dx.doi.org/10.1007/s40242-021-1038-1; https://link.springer.com/10.1007/s40242-021-1038-1; https://dx.doi.org/10.1007/s40242-021-1038-1; https://link.springer.com/article/10.1007/s40242-021-1038-1; http://sciencechina.cn/gw.jsp?action=cited_outline.jsp&type=1&id=7216385&internal_id=7216385&from=elsevier
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