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Influence of CeO nanoparticles synthesized from plant extracts on the catalytic performance of isolated catalase from carrots

Emergent Materials, ISSN: 2522-574X, Vol: 5, Issue: 3, Page: 673-682
2022
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Article Description

This paper describes the green synthesis of well-crystalline CeO nanoparticles (NPs) using the extract of lemon leaves (Citrus limon) and efficiently used as substituted catalase to catalyze the various catalase enzyme. The spherical particles along with typical face-center cubic crystalline phase were observed for CeO NPs which were further characterized in terms of compositional, structural, surface, and enzyme catalytic properties. Catalase was isolated from carrots (Daucus carota). The optimum pH and temperature for catalase activity was found to be 6.0 and 35 °C, respectively. K value was found to be 17 mM calculated from the double reciprocal plot. The enzyme was found to be an eco-friendly reagent for oxidation of toxic compounds like phenols, acetaldehydes, acetic acid, and sodium nitrite. CeO nanoparticles biomimic catalase and degrade HO efficiently. Optimum pH and temperature for catalase-like activity by CeO nanoparticles were found to be 6.0 and 30 °C, respectively. K value was found to be 14.96 mM from the double reciprocal plot. Comparative studies of catalase and CeO nanoparticles with respect to kinetic parameters like pH, temperature, and Michaelis–Menten constant K have been established.

Bibliographic Details

Jitendra Kumar Sharma; Pratibha Srivastava; Meera Yadav; Anindita Hazarika; Hardeo Singh Yadav; Nene Takio; Sadia Ameen; M. Shaheer Akhtar

Springer Science and Business Media LLC

Materials Science; Energy; Environmental Science

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