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A comparison of kombucha SCOBY bacterial cellulose purification methods

SN Applied Sciences, ISSN: 2523-3971, Vol: 2, Issue: 2
2020
  • 64
    Citations
  • 0
    Usage
  • 275
    Captures
  • 2
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    64
    • Citation Indexes
      64
  • Captures
    275
  • Mentions
    2
    • References
      2
      • Wikipedia
        2

Article Description

Kombucha SCOBY pellicle formed as a waste product in the kombucha tea fermentation industry can be used as a potential source for bacterial cellulose. We have compared four simple, scalable purification methods for the purification of this jelly like cellulosic matrix containing bacteria, yeast cells, proteins and polyphenols as impurities. The method using two successive 1.0 M NaOH washings at 90 °C, followed by the treatment with 1.5% (w/w) aq. NaOCl for 2 h, was the most effective method and gave the purified cellulose sample with the highest leucometer whiteness value of 81.4 ± 4.8. The purified cellulose samples were analyzed using FT-IR, TGA, SEM, EDX, X-ray and water absorption at room temperature.

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