Molecular cloning of complementary DNA: Preparation of a plasmid vector with low transformation background
Analytical Biochemistry, ISSN: 0003-2697, Vol: 129, Issue: 1, Page: 249-252
1983
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- 3Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
A simple method that allows the rapid preparation of oligo dG-tailed plasmid vectors is presented. The procedure involves purification of the tailed molecules by hybridization to oligo dC-cellulose followed by a stepwise thermal elution. The resulting plasmid is virtually devoid of transformation activity in the absence of oligo dC-tailed DNA fragments. It allows construction of cDNA libraries with as low as 1% of colonies harboring wild-type plasmids.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0003269783900775; http://dx.doi.org/10.1016/0003-2697(83)90077-5; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0021103406&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/6687985; https://linkinghub.elsevier.com/retrieve/pii/0003269783900775; http://dx.doi.org/10.1016/0003-2697%2883%2990077-5; https://dx.doi.org/10.1016/0003-2697%2883%2990077-5
Elsevier BV
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