Sedimentation equilibrium studies of human chymotrypsin II and bovine δ-chymotrypsin
Archives of Biochemistry and Biophysics, ISSN: 0003-9861, Vol: 154, Issue: 1, Page: 160-165
1973
- 2Citations
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- Citations2
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Article Description
Sedimentation equilibrium experiments indicate that neither human chymotrypsin II nor bovine δ-chymotrypsin molecules undergo association in the pH range 3–5 where dimerization occurs with α-chymotrypsin. The weight-average molecular weights of human chymotrypsin II and δ-chymotrypsin in a pH 4.4 0.1 ionic strength buffer are 26,200 and 26,400, respectively, using the measured partial specific volumes of 0.722 and 0.727 ml/g at 25 °C. Number-average molecular weight calculations also support the presence of monomeric species at this pH. In the pH range 6–7.6 where sedimentation velocity studies have shown that δ-chymotrypsin associates at concentrations above 3 mg/ml, no association was observed for either the human chymotrypsin II or bovine δ-chymotrypsin in the sedimentation equilibrium experiments where protein concentrations were below 1.2 mg/ml. These studies provide additional evidence that human chymotrypsin II is similar to bovine δ-chymotrypsin.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0003986173900441; http://dx.doi.org/10.1016/0003-9861(73)90044-1; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0015544251&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/4734721; https://linkinghub.elsevier.com/retrieve/pii/0003986173900441; http://dx.doi.org/10.1016/0003-9861%2873%2990044-1; https://dx.doi.org/10.1016/0003-9861%2873%2990044-1
Elsevier BV
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