Uptake of [ 3 h]cholesterol from low density lipoprotein by cultured human fibroblasts
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, ISSN: 0005-2760, Vol: 711, Issue: 2, Page: 281-289
1982
- 23Citations
- 1Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations23
- Citation Indexes23
- 23
- CrossRef21
- Captures1
- Readers1
Article Description
The uptake of [ 3 H]cholesterol from low density lipoprotein (LDL) was studied in LDL receptor-positive and receptor-negative human fibroblasts. In both cell lines the uptake depended upon temperature, time of incubation and the concentration of LDL in the medium. Although the incorporation of 125 I-labeled LDL was minimal after 2 h of incubation in the receptor-negative (homozygous familial hypercholesterolemia, FH) cells, the uptake of [ 3 H]cholesterol was only slightly less than that of the receptor-positive (WI-38) cells. With longer periods of incubation, a larger difference in labeled cholesterol incorporation was observed; this appeared to be due to a continued accumulation of the steroid in the WI-38 cells. After 8 and 24 h of incubation, some of the [ 3 H]cholesterol was present as the ester in the WI-38 cells, but not the FH cells. Modified (reduced and methylated) LDL did not enter WI-38 cells by the receptor-mediated pathway during 2 h of incubation, as indicated by 125 I uptake. [ 3 H]Cholesterol uptake, however, was not significantly different from modified and unmodified LDL. While experiments indicated that significant amounts of cholesterol moved rapidly from LDL to cultured cells with a dependence on time and LDL concentration, no increase in total cell cholesterol was detected in either cell line. FH cells contained less total cholesterol and had a higher 3 H specific activity than the WI-38 cells. These data suggest that there may be important mechanisms in addition to the LDL pathway for the movement of lipids into cells.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0005276082900376; http://dx.doi.org/10.1016/0005-2760(82)90037-6; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0020332755&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/6284241; https://linkinghub.elsevier.com/retrieve/pii/0005276082900376; http://dx.doi.org/10.1016/0005-2760%2882%2990037-6; https://dx.doi.org/10.1016/0005-2760%2882%2990037-6
Elsevier BV
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