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Isolation of stable ribosomal RNA from whole cells of Chlamydomonas reinhardtii

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, ISSN: 0005-2787, Vol: 269, Issue: 2, Page: 259-264
1972
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Sonication, rather than lysis, of cells in the presence of diethylpyrocarbonate and Mg 2+ as nuclease inhibitors has resulted in the isolation of stable high molecular weight ribosomal RNA from Chlamydomonas reinhardtii. Preparations are nuclease free and show excellent conservation of the previously thought labile 23-S rRNA species.

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