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Hemoglobin covalently bridged across the polyphosphate binding site

Biochemical and Biophysical Research Communications, ISSN: 0006-291X, Vol: 63, Issue: 4, Page: 1123-1129
1975
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Article Description

Hemoglobin has been cross-linked covalently by reaction with 2-nor-2-formylpyridoxal 5′-phosphate followed by reduction with sodium borohydride. It is shown that a bridge is formed across the polyphosphate binding site between the two β chains. The modified hemoglobin binds oxygen cooperatively with a greatly decreased affinity demonstrating that the cross-link stabilizes the deoxy conformation but does not prevent the conformational change associated with oxygenation.

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