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Differences in the cellular location of substances endocytosed by rat liver as observed from the distribution patterns obtained after isopycnic centrifugation in a sucrose gradient

Biochemical and Biophysical Research Communications, ISSN: 0006-291X, Vol: 136, Issue: 2, Page: 504-509
1986
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We have investigated the distribution of several substances endocytosed by rat-liver, after isopycnic centrifugation in a sucrose gradient of the MLP fractions ( de Duve, Pressman, Gianetto, Wattiaux and Applemans (1955) Biochem. J. 63, 604–617) isolated at increasing times after injection. It has been observed that there are changes in the distribution pattern with time depending on whether the substance is taken up by parenchymal or sinusoidal cells. The results suggest that centrifugation experiments can be informative with respect to the cellular location of a molecule endocytosed by the liver.

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