PlumX Metrics
Embed PlumX Metrics

Tauroursodeoxycholic acid inhibits the cytosolic Ca ++ increase in human neutrophils stimulated by formyl-methionyl-leucyl-phenylalanine

Biochemical and Biophysical Research Communications, ISSN: 0006-291X, Vol: 171, Issue: 3, Page: 1115-1121
1990
  • 18
    Citations
  • 0
    Usage
  • 1
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

The effect of the cytoprotective bile acid tauroursodeoxycholic acid (TUDCA) on basal cytosolic free Ca ++ (Ca ++ ) i and receptor-mediated (Ca ++ ) i increase was studied in human polymorphonuclear neutrophils using the fluorescent dye quin2. Basal levels of (Ca ++ ) i were 96 ± 6 nmol/1 (mean ± SEM, n=48). TUDCA and its cytotoxic epimer taurochenodeoxycholic acid (TCDCA) at 500 μmol/l increased (Ca ++ ) i by 31 ± 12 and 27 ± 7 nmol/l, respectively (n=6, p<0.05). Stimulation of neutrophils with the chemotactic tripeptide N-formyl-methionyl-leucyl-phenylalanine (FMLP; 10 −7 mol/l) induced a (Ca ++ ) i increase of 200 ± 32 nmol/l which was inhibited after preincubation with TUDCA (500 μmol/l) or TUDCA-TCDCA (500 μmol/l, each) by 60.1% and 59.5%, respectively, but not with TCDCA (500 μmol/l) alone. The inhibitory effect of TUDCA on FMLP-induced (Ca ++ ) i increase was strongly concentration-dependent and was nearly complete at 1000 μmol/l. Since (Ca ++ ) i is discussed as a mediator of cellular injury we hypothesize that TUDCA may exert its protective effects at least partly via inhibition of (Ca ++ ) i -mediated cytotoxic processes.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know