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The solubilization of membrane-bound (Na + + K + )-ATPase from rat skeletal muscle

Biochemical Medicine, ISSN: 0006-2944, Vol: 19, Issue: 3, Page: 344-350
1978
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Article Description

A (Na + + K + )-ATPase (EC 3.6.1.3.) of skeletal muscle was solubilized by treatment with Lubrol WX. Maximum extraction of this enzyme was obtained at a Lubrol WX:protein ratio of 2.5:1. The supernatant fraction of the Lubrol WX extract obtained by centrifugation at 140,000 g for 45 min after NaI treatment had a higher specific activity and greater ouabain sensitivity than that of the NaI-extracted frozen muscle homogenate, which had not been extracted with Lubrol WX.

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