The solubilization of membrane-bound (Na + + K + )-ATPase from rat skeletal muscle
Biochemical Medicine, ISSN: 0006-2944, Vol: 19, Issue: 3, Page: 344-350
1978
- 1Citations
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Article Description
A (Na + + K + )-ATPase (EC 3.6.1.3.) of skeletal muscle was solubilized by treatment with Lubrol WX. Maximum extraction of this enzyme was obtained at a Lubrol WX:protein ratio of 2.5:1. The supernatant fraction of the Lubrol WX extract obtained by centrifugation at 140,000 g for 45 min after NaI treatment had a higher specific activity and greater ouabain sensitivity than that of the NaI-extracted frozen muscle homogenate, which had not been extracted with Lubrol WX.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0006294478900340; http://dx.doi.org/10.1016/0006-2944(78)90034-0; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0018147857&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/150271; https://linkinghub.elsevier.com/retrieve/pii/0006294478900340; http://dx.doi.org/10.1016/0006-2944%2878%2990034-0; https://dx.doi.org/10.1016/0006-2944%2878%2990034-0
Elsevier BV
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