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Protein methylation in normal and cystic fibrosis fibroblasts

Biochemical Medicine, ISSN: 0006-2944, Vol: 31, Issue: 1, Page: 104-111
1984
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Article Description

Human-cultured fibroblasts contain protein methylase activities. These activities were determined and the enzymatic products were identified after acid hydrolysis of the protein substrate for protein methylases I (arginine) and III (lysine) and by organic solvent extraction of the methanol produced by alkaline treatment of the protein substrate (for the protein methylase II). A methylation of histidine residues of proteins occurs in cultured fibroblasts. Protein methylase activities were unmodified in the cystic fibrosis fibroblasts as compared to the control cells.

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